MycoBlue Mycoplasma Detector - 50 rxn
The MycoBlue Mycoplasma Detector offers a fast, simple, and highly accurate method for detecting mycoplasma contamination in cell cultures—without the need for PCR, qPCR, or electrophoresis.
Buy here from the manufacturer.
Just add 1 µl of cell culture supernatant to the reaction mix, incubate at 60°C for 1 hour, and visually inspect the result. No specialized equipment is required, making it ideal for routine use directly in the cell culture lab.
Key Advantages
- Fast: Results in just 1 hour at 60°C
- Simple: Add sample directly—no DNA extraction or pretreatment needed
- Accurate: Detection performance comparable to qPCR, superior to traditional PCR
- Contamination-Free: Eliminates the need for electrophoresis, reducing aerosol-related false positives
- Inhibitor-Tolerant: More resistant to culture medium inhibitors—reduces false negatives
Broad Detection Range
Validated to detect 28 species of mycoplasma, including the 8 most common contaminants in cell culture. Compatible with a wide range of suspension and adherent cell lines, including:
- CHO
- Vero
- Hybridoma
- Sf9
- HEK293
Suitable for use with most standard culture media.
Applications
Ideal for routine testing in:
- Biopharmaceutical production
- Vaccine and monoclonal antibody manufacturing
- Cell therapy and embryo laboratories
- Academic and industrial research labs